With energy prices reaching historical highs, biodiesel as an alternative fuel is increasingly attracting attention. Currently, biodiesel is made from a variety of feedstocks, including pure vegetable oils, waste cooking oils, and animal fat; however, the limited supply of these feedstocks impedes the further expansion of biodiesel production. Microalgae have long been recognized as potentially good sources for biofuel production because of their high oil content and rapid biomass production.